Fig. 3

Effects of 200 μM FFA C8:0 on biological behavior and KLF7 expression level of PCa cells. A, F CCK8 assay was used to detect the proliferation abilities of PC3 cells (A) and 22RV1 cells (F) with different concentrations of FFA C8:0. B, G The effects of 200 μM FFA C8:0 on the colony formation ability of PC3 cells (B) and 22RV1 cells (G) were detected by plate Colony assay. C, H The effects of 200 μM FFA C8:0 on the invasion abilities of PC3 cells (C) and 22RV1 cells (H) were detected by Transwell assay (200 ×). D, I The effects of 200 μM FFA C8:0 on the migration abilities of PC3 cells (D) and 22RV1 cells (I) were detected by Transwell assay (200 ×). E, J The effects of 200 μM FFA C8:0 on the healing abilities of PC3 cells (E) and 22RV1 cells (J) were detected by scratch experiment (40 ×). K The mRNA expression levels of KLF7, IL-6, p21 and MMP2 in PC3 cells stimulated by 200 μM FFA C8:0 were detected by qRT-PCR. L The protein expression levels of KLF7, IL-6, p21 and MMP2 in PC3 cells stimulated by 200 μM FFA C8:0 were detected by Western Blot. M The secretion level of IL-6 in PC3 cells stimulated by 200 μM FFA C8:0 were detected by ELISA. t test, *P < 0.05, **P < 0.01 the difference was statistically significant