Fig. 8

IHC analysis to study the expression of KRT7, KRT19 and SRI in the GBC cases and control group. (A) Representative IHC images showing the expression of KRT7, KRT19 and SRI in controls, LN Negative GBC and LN Positive GBC cases. IHC was performed on formalin-fixed paraffin-embedded (FFPE) individual tissue sections of 15 controls (GSD cases with no dysplasia), 15 LN Negative GBC cases and 15 LN Positive GBC cases. The IHC results showed that the expression of KRT7 was found to be ‘positive’ in 100% GSD cases, 33% LN negative GBC and 86% LN positive GBC. The expression of KRT19 was found to be ‘positive’ in 100% GSD cases, 93% LN negative GBC and 100% LN positive GBC. The expression of SRI was found to be ‘positive’ in 6% GSD cases, 20% LN negative GBC and 60% LN positive GBC. (B) The statistical analysis between LN positive vs LN negative GBC showed a significant difference in KRT7 and SRI expression (p value 0.002 and 0.02 respectively) while KRT19 did not show any significant difference in expression. The p values ≤ 0.05, ≤ 0.01 are marked with ‘*’ and ‘**’ respectively. The scale bar is shown as white line. IHC scoring is shown in Methodology section 2.8. GSD- Gallstone disease, LN- Lymph node