Fig. 3

Proliferated pre-B cells expressed GSDMD during the progression of B-ALL. A, B. B cell clusters were extracted from initial diagnosis and relapse, respectively. Cell clustering with cell type annotation identified pre-B cells, immature B cells, plasma cells, and lymphoid cells in both diagnosis (A) and relapse (B). C, D. Sample annotation assists in distinguishing B cells from different B-ALL patients at the diagnosis (C) and relapse (D) stages. E. T-SNE plots profiled the expression of MME, VPREB1, TMEM173, and GSDMD in B cells from diagnosis. F. T-SNE plots exhibited the expression of MME, CTGS, TMEM173, and GSDMD in B cells from relapse. G. Pseudo-time analysis revealed the expression of GSDMD with the developmental trajectory of B cells in both the initial diagnosis and relapse stage. The arrows represented the direction of GSDMD attenuation. H, I. Comparison of specific genes (NFKB1A, ATK, CD22, CD19, and BTK) with GSDMD in diagnosis (H) and relapse (I) by violin plots.