Fig. 2

Expression levels of TMEM173 and GSDMD were decreased in pre-B cell clusters. A-D. Transcriptome features of TMEM173 in BM B cells. B cell clusters were extracted from total BM cells. (A) Cell clustering with sample annotation identified the different origins of B cells. (B) Cell type annotation classified B cells into pre-B cells, immature B cells, plasma cells, multi-lymphoid progenitor cells, and lymphoid cells. (C) T-SNE plots profiled typical marker genes in B cells, including CD19, CD24, IGHM, IGHD, TMEM173, and GSDMD. (D) The expression levels of typical marker genes were compared by dot plot. E-H. Transcriptome features of TMEM173 in B cells from B-ALL patients. B cell clusters were extracted from patients’ BM cells. (E) Cell type annotation classified B cells into 5 categories. (F) Sample annotation identified B cells from different patients. (G) T-SNE plots profiled typical marker genes in B cells, including MME, MZB1, IGLC2, IGHD, TMEM173, and GSDMD. (H) The expression levels of typical marker genes were compared by dot plot.