Fig. 1

The transcriptome profiles of BM cells in B-ALL patients were different from healthy donors. A, B. Total RNA and protein were extracted from PBMCs of each individual (N1-N3 = healthy donors; T1-T6 = B-ALL patients). (A) Relative mRNA levels of TMEM173 were increased in PBMCs from 6 B-ALL patients (p < 0.05, Reference gene = GAPDH). (B) Quantitative (upper) and relative levels (lower) of TMEM173 protein in PBMCs of 6 B-ALL patients (p < 0.05, Reference gene = GAPDH). 3 asterisk-marked samples (T4-T6) were obtained from high-risk B-ALL patients. Representative images were extracted from the full-length PVDF membranes around the molecular weight of TMEM173 and GAPDH. The original images were presented in Supplementary Fig. 6. C-E. ScRNA-seq data of 2 healthy donors and 2 B-ALL patients (both diagnosis and recurrence) was fully merged by the Harmony package. (C) Cell clustering with cell type annotation identified cell compositions in BM cells. (D) Differential expression of TMEM173 between B-ALL patients and healthy controls was profiled in t-SNE plots by using the Split-by function. (E) Violin plot compared the expression levels of TMEM173 in different types of BM cells. F-I. BM cells from B-ALL patients (both diagnosis and recurrence) and healthy donors were merged separately. (F). Cell clustering with cell type annotation displayed cell compositions in B-ALL (left) and healthy BM (right). (G) Volcano plots compared the DEGs between B cells and all others in B-ALL (left) and healthy BM (right). Heatmap analysis exhibited the most significant DEGs of each cell cluster in B-ALL patients (H) and healthy donors (I). Vertical bars in A and B indicated mean ± SD from three separate experiments. The statistical significance was determined by the unpaired two-tailed T-test. ^*p < 0.05, ^**p < 0.01.