Fig. 3

Overexpressed AC010883.5 promoted proliferation and progression in C-33A cells. A. qRT-PCR analyses of AC010883.5 expression in C-33A cells transfected with the oeAC010883.5 vector. B. CCK-8 analysis of viable C-33A cells under AC010883.5 treatment (overexpressed vs. control). C. BrdU incorporation analysis of proliferative C-33A cells under AC010883.5 treatment (overexpressed vs. control). D. Quantification of flow cytometry results in C33-A cells under AC010883.5 treatment (overexpressed vs. control). E. Flow cytometry analysis of apoptotic C-33A cells under AC010883.5 treatment (overexpressed vs. control). F. Transwell migration analysis of migrative C-33A cells under AC010883.5 treatment (overexpressed vs. control). G. Transwell invasion analysis of invasive C-33A cells under AC010883.5 treatment (overexpressed vs. control). H. Wound healing analysis of migrative C-33A cells under AC010883.5 treatment (overexpressed vs. control). I. Western blots of E-cadherin and N-cadherin in C-33A cells under AC010883.5 treatment (overexpressed vs. control). Abbreviations: qRT-PCR, quantitative real-time polymerase chain reaction; CCK-8, Cell Counting Kit‐8; BrdU, 5‐Bromo-2'-deoxyuridine