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Fig. 2 | BMC Cancer

Fig. 2

From: AC010883.5 promotes cell proliferation, invasion, migration, and epithelial-to-mesenchymal transition in cervical cancer by modulating the MAPK signaling pathway

Fig. 2

Silencing AC010883.5 suppressed proliferation and progression in SiHa cells. A. qRT-PCR analysis of AC010883.5 expression in SiHa cells transfected with shRNA-2 and shRNA-3. B. CCK-8 analysis of viable SiHa cells under AC010883.5 treatment (silenced vs. control). C. BrdU incorporation analysis of proliferative SiHa cells under AC010883.5 treatment (silenced vs. control). D. Cell cycle quantification of SiHa cells using flow cytometry. E. Flow cytometry analysis of apoptotic SiHa cells under AC010883.5 treatment (silenced vs. control). F. Transwell migration analysis of migrative SiHa cells under AC010883.5 treatment (silenced vs. control). G. Transwell invasion analysis of invasive SiHa cells under AC010883.5 treatment (silenced vs. control); H. Wound healing analysis of migrative SiHa cells under AC010883.5 treatment (silenced vs. control). I. Western blots of E-cadherin and N-cadherin in SiHa cells under AC010883.5 treatment (silenced vs. control). N = 3; Abbreviations: qRT-PCR, quantitative real-time polymerase chain reaction; shRNA, short hairpin RNA; CCK-8, Cell Counting Kit‐8; BrdU, 5‐Bromo-2'-deoxyuridine. *P < 0.05, **P < 0.01, and ***P < 0.001

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BMC Cancer

ISSN: 1471-2407

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