Fig. 1

The relative expression and subcellular location of AC010883.5. A. qRT-PCR analysis of AC010883.5 expression in CC and adjacent cervical tissues from ten patients. B. qRT-PCR analyses of AC010883.5 expression in four CC cell lines and one normal cell line. C. An investigation of the subcellular location of AC010883.5 in SiHa and C-33A cells by qPCR. The original magnification was 400 × . D. AC010883.5 expression in SiHa cells transfected with si-NC or one of four experimental siRNAs. E. CCK-8 analysis of AC010883.5 expression in SiHa cells transfected with si-NC or siRNA 1 to 4. The error bars represent the mean ± SD of at least three experiments. N = 3. All qPCR results were normalized to GAPDH. *P < 0.05, **P < 0.01, and ***P < 0.001. Abbreviations: qRT-PCR, quantitative real-time polymerase chain reaction; CC, cervical cancer; si-NC, negative control siRNA; siRNA, small interfering RNA; CCK-8, Cell Counting Kit‐8; DAPI, 4′,6‐diamidino‐2‐phenylindole