Fig. 5

Differential uptake of calcium levels to induce blast cell differentiation. A Flow cytometric analysis of effect of amlodipine (membrane calcium channel blocker) on intracellular calcium levels. Cells were treated with esculetin alone or in combination with amlodipine for 72 h and stained with Fluo-4AM and subjected to flow cytometric analysis. A left shift in the fluorescence intensity demonstrate enhanced intracellular calcium levels in response to esculetin treatment. Relative fold change of three independent experiments were shown in lower panel. (*p ≤ 0.05; **p ≤ 0.02). B CD11b expression following 96 h of esculetin treatment alone or in combination with amlodipine. Relative CD11b expression of three independent experiments were shown in lower panel. (*p ≤ 0.05; **p ≤ 0.02). C Effect of TMB-8 (endoplasmic reticulum IP3R calcium channel blocker) on intracellular calcium levels following esculetin alone or in combination with TMB8 treatment. Cells were processed as mentined for amlodipine treatment. Relative fold change of three independent experiments were shown in lower panel. D CD11b expression alone or in combination with 96 h of esculetin treatment. Lower panel indicate relative CD11b expression of three independent experiments. (*p ≤ 0.05; **p ≤ 0.02)