Fig. 3

Terminal differentiation potential of esculetin. A Neturophilic (Left panel) and monocytic (right panel) specific surface marker analysis using flow cytometry to trace the leneage commitment following 96 h of 100 μM esculetin treatment. Data represents three independent experiments. (*p ≤ 0.05; **p ≤ 0.02). B Microscopic analysis of Wright-Giemsa-stained cells following 96 h of esculetin treatment (magnification: 60X). A total of 500 randomly selected cells were analyzed for nuclear alterations and appearance of vacuolation. Relative percentage alteration which includes bilobed, multilobed, acentric nucleus, cells with lower nucleocytoplasmic (N/C) ratio and/or presence of vacuolation were plotted (right panel). Data represents three independent experiments. (*p ≤ 0.05; **p ≤ 0.02)