Fig. 1
Experimental design. 14 wk. old BALB/c female mice were injected with 106 isogenic 4T1 murine breast cancer cells labeled with GFP. After 20 days, mice were again anesthetized with isoflurane and primary tumors were excised en-block. Artificial ε-PCL implants were implanted subcutaneously on the contralateral side immediately after the tumor excision. After 10 more days mice were sacrificed and implants along with the lung tissue were harvested for flow cytometric analysis for quantification of metastatic burden and their differentiation state