Fig. 6

SPRYD4 plays a key role in cell cycle and apoptosis in CCA cells. A Flow cytometry showed over-expression of SPRYD4 triggered G2/S blockade in HUCCT1 cells, while depletion of SPRYD4 increased the percentage of S phase in CCLP-1 cells. B Correlations of SPRYD4 expression and CDK2, CCNA2 shown by GEPIA web server. C RT-qPCR validated the expression of CDK2 and CCNA2 was down-regulated in HUCCT1/SPRYD4-OV cells. D Flow cytometry showed that SPRYD4 over-expression increased the percentage of apoptotic HUCCT1 cells, while SPRYD4 deletion reduced the percentage of apoptotic CCLP-1 cells. E Correlations of SPRYD4 expression and BCL-2, BIRC5 shown by GEPIA web server. F RT-qPCR validated the expression of BCL-2 and BIRC5 (Survivin) was down-regulated in CCLP1/SPRYD4-KD cells. P-values were assessed using two-tailed t-tests in A, C, D and F. Spearman’s correlation was performed in B and E. All *** P-value < 0.001