Fig. 4

SPRYD4 participates in CCA cells proliferation and migration. A Rt-qPCR showed SPRYD4 was highly expressed in CCLP-1 cells, while relatively low-expressed in HUCCT1 cells among CCA cell lines. B-C RT-qPCR were used to detect the over-expression of SPRYD4 in HUCCT1 cells (B) and the knock-down of SPRYD4 in CCLP-1 cells (C) after transfection. D-E CCK-8 assay (D) and colony formation assay (E) showed that SPRYD4 over-expression reduced the proliferation of HUCCT1 cells, while SPRYD4 knock-down promoted the proliferation of CCLP-1 cells. F-G Transwell assay (F) and wound-healing assay (G) showed that SPRYD4 over-expression reduced the migration of HUCCT1 cells, while SPRYD4 knock-down promoted the migration of CCLP-1 cells. Scale bars: 100 μm. P-values were assessed using two-tailed t-tests and ANOVA followed by Dunnett’s tests for multiple comparison in B-G. All *** P-value < 0.001