Fig. 1

SPRYD4 is low-expressed in CCA. A Low-expression of SPRYD4 in CCA was identified in GEPIA database. B Down-regulation of SPRYD4 was identified in CCA in four individual GEO datasets (GSE26566, GSE32225, GSE32958 and GSE76311). C The mRNA over-expression levels of SPRYD4 were identified in CCA tissues and normal bile duct tissues of 20 samples. D Representative images of SPRYD4 staining in CCA specimens and normal bile duct tissues. E Immunohistochemistry staining showed the protein levels of SPRYD4 were down-regulated in CCA tissues. Scale bars: 200 μm. P-values were determined by Non-parametric Mann–Whitney U-test in A-B. P-values were assessed by two-tailed t-tests in C and E. All * P-value < 0.05, ** P-value < 0.01, *** P-value < 0.001