Fig. 5
From: Chidamide enhances cytotoxicity of doxorubicin by promoting autophagy and apoptosis in breast cancer
CHI treatment increased the sensitivity of MCF-7 cells to DOX, as indicated by decreased tumor growth. A Illustrative images of nude mouse xenografts regarding MCF-7 cells. B, C Tumor volumes in mice were detected every 5 days. Results are expressed as means ± SD. ***P < 0.001 vs. CHI. D, E Immunohistochemical detection showed the expression of Ki67 in the tumor tissues. Results are expressed as means ± SD. ***P < 0.001 vs. NC. Scal bar, 100 μm. F, G TUNEL staining showed the apoptosis in tumor tissues. Data are expressed as means ± SD. ***P < 0.001 vs. NC. Scal bar, 100 μm. H, I Immunofluorescence detection showed the expression of autophagy related protein LC3 in the tumor tissues. Results are expressed as means ± SD. ***P < 0.001 vs. NC. Scal bar, 20 μm