Fig. 8

Overexpression of DTL may cause DNA damage, genomic instability and apoptosis. A Western blot for γ-H2AX in HEK293-Ctrl and HEK293-DTL cells. B Representative IF images of γ-H2AX staining in HEK293-Ctrl and HEK293-DTL cells (L) and statistical analysis of the percentage of γ-H2AX-positive cells against DAPI staining (R) (t-test *P < 0.05, **P < 0.01, ***P < 0.001), (γ-H2AX: red, DAPI: blue, 20X). C-D Representative IF images of p-p53(S15) and cleaved-caspase-3 staining in HEK293-Ctrl and HEK293-DTL cells (L) and statistical analysis of the percentage of p-p53(S15)-positive cells and cleaved-caspase-3–positive cells against DAPI staining (R) (t-test *P < 0.05, **P < 0.01, ***P < 0.001), (p-p53(S15) and cleaved-caspase-3: red, DAPI: blue, 20X). E Representative images of chromosome in HEK293-Ctrl and HEK293-DTL cells (L) and statistical analysis of the percentage of chromosome breakages (R) (t-test *P < 0.05, **P < 0.01, ***P < 0.001). F Representative images of chromosome slide preparation in HEK293-Ctrl and HEK293-DTL cells (L) and statistical analysis of the percentage of micronucles(t-test *P < 0.05, **P < 0.01, ***P < 0.001)