Fig. 7
Overexpression of DTL promotes cell proliferation and regulates the cell cycle. A HEK293-Ctrl and HEK293-DTL cells were observed in Live cell imaging system 20 h and taken a picture every 30minuts. Images of the first and last were above (L) and the number of cells were analyzed (R). B HEK293-Ctrl and HEK293-DTL cells were detected cell cycle by FC assays. C Representative images of BrdU staining in HEK293-Ctrl and HEK293-DTL cells (L) and statistical analysis of the percentage of BrdU-positive cells against DAPI staining (R) (t-test *P < 0.05, **P < 0.01, ***P < 0.001), (BrdU: red, DAPI: blue, 20X). D Representative images of cyclinB1 staining in HEK293-Ctrl and HEK293-DTL cells (L) and statistical analysis of the percentage of cyclinB1-positive cells against DAPI staining (R) (t-test *P < 0.05, **P < 0.01, ***P < 0.001), (cyclinB1: red, DAPI: blue, 20X)