Fig. 2

XBP1-deficient cells show reduced proliferation and accumulate in G1 of cell cycle. A MCF7 control and XBP1 knockout sub-clones of MCF7 cells (#9 and #16) were plated in 96-well plate. Line graphs show change in O.D at 490 nm. Data presented is mean ± SD from six replicates (n = 3) (B) MCF7 control and XBP1 knockout sub-clones were seeded in 6-well plate (1000 cells/well) and maintained for 14 days. Crystal violet stained colonies are shown. Lower panel shows the quantification of size and number of colonies per well. Data shown is mean ± S.D (n = 3). *p < 0.05, two tailed unpaired t-test compared with controls. C Cell cycle analysis of control and XBP1 KO (#16) MCF7 cells using PI staining followed by flow cytometry. Representative data of three independent analyses is shown