Fig. 1

LncRNA EGFR-AS1 binds to TUBA1C protein. A LncRNA EGFR-AS1 expression was detected by qRT-PCR in GC cell lines(HGC-27, BGC-823, MGC-803, MKN-45 and SGC-7901) and the normal gastric cell line (GES-1). B Schematic representation of the plasmid contained EGFR-AS1 or its antisense sequence. C The expression of the protein obtained by the pull-down method in HGC-27 cells was detected by silver staining. D Top 10 reliable proteins identified by mass spectrometry E TUBA1C was identified as A EGFR-AS1-binding protein. F RNA-pulldown followed by Western blot analysis to identify the EGFR-AS1–TUBA1C interaction G The effects of EGFR-AS1 overexpression and knockdown on TUBA1C protein expression. (The blots were cut prior to hybridisation with antibodies.) (oe = overexpression, NC = negative control).Data are mean ± SD. (Student’s t test, ***p < 0.001)