Fig. 5
Effects of exposure to combined G-CSF + cisplatin on germ cell numbers in mouse pre-pubertal testicular tissues at 240 hrs post-exposure. Representative images of IF staining for expression of spermatogonial (MVH, purple) and SSC (PLZF, green) protein markers in control (A), 0.5 μg/ml cisplatin (B) or 100 ng/ml G-CSF + cisplatin exposed mouse pre-pubertal testicular tissues from Regimen 3 (C). White arrows point to MVH+vePLZF+ve cells, yellow arrowheads MVH+vePLZF-ve cells at the basement membrane and white arrowheads to MVH+vePLZF-ve cells in the middle of the tubules. Quantification of total germ cell (D) and putative SSC (E) numbers per tubular area (mm2). Each set of coloured points represents an individual litter (n = 4). Data presented as mean ± SEM and analysed using one-way ANOVA (****p < 0.0001)