Table 2 Antibodies used for Western blot (WB), immunocytochemistry (ICC) or immunohistochemistry (IHC) and immunofluorescence (IF).

WB

N-cadherin

FS1,

TCam-2

Mouse monoclonal antibody,

33-3900, Invitrogen

1:500

Goat anti-mouse IgG-HRP,

sc-2005, Santa Cruz, 1:5000

Connexin 43

FS1,

TCam-2

Rabbit polyclonal antibody,

3512, NEB * / Cell Signaling

1:1000

Goat anti-rabbit IgG-HRP,

sc-2004, Santa Cruz, 1:5000

Connexin 45

FS1,

TCam-2

Rabbit polyclonal antibody,

T. Steinberg, St. Louis [62–64]

1:1000

Goat anti-rabbit IgG-HRP,

sc-2004, Santa Cruz, 1:5000

Beta-actin

FS1,

TCam-2

Mouse monoclonal antibody,

sc-47,778, Santa Cruz

1:5000

Goat anti-mouse IgG-HRP,

sc-2005, Santa Cruz, 1:5000

ICC/ IHC

N-cadherin

FS1,

TCam-2

Mouse monoclonal antibody,

33-3900, Invitrogen

1:1000 ^†

1:2000 ^‡

HRP Labelled Polymer Anti-Mouse,

K4001, Dako, ready to use

Testis

Rabbit polyclonal antibody, ab76057, Abcam

1:1500 ^§

1:3000 \( ^\parallel \)

HRP Labelled Polymer Anti-Rabbit,

K4003, Dako, ready to use

Connexin 43

FS1,

TCam-2

Rabbit polyclonal antibody,

3512, NEB * / Cell Signaling

1:100

HRP Labelled Polymer Anti-Rabbit,

K4003, Dako, ready to use

Testis

Rabbit polyclonal antibody,

3512, NEB * / Cell Signaling

1:500

HRP Labelled Polymer Anti-Rabbit,

K4003, Dako, ready to use

Connexin 45

FS1,

TCam-2

Rabbit polyclonal antibody,

T. Steinberg, St. Louis [62–64]

1:6000

HRP Labelled Polymer Anti-Rabbit,

K4003, Dako, ready to use

Testis

Rabbit polyclonal antibody,

CX45B12-A, Alpha Diagnostic

1:1000

HRP Labelled Polymer Anti-Rabbit,

K4003, Dako, ready to use

IF

N-cadherin

FS1,

TCam-2

Mouse monoclonal antibody,

33-3900, Invitrogen

1:1000

Alexa Fluor 488 anti-Mouse,

A-11,001, Invitrogen, 1:5000

Connexin 43

FS1,

TCam-2

Rabbit polyclonal antibody,

3512, NEB * / Cell Signaling

1:100

Alexa Fluor 488 anti-Rabbit,

A-11,008, Invitrogen, 1:5000

Connexin 45

FS1,

TCam-2

Rabbit polyclonal antibody

T. Steinberg, St. Louis [62–64]

1:6000

Alexa Fluor 488 anti-Rabbit,

A-11,008, Invitrogen, 1:5000