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Fig. 5 | BMC Cancer

Fig. 5

From: Analysis of connexin 43, connexin 45 and N-cadherin in the human sertoli cell line FS1 and the human seminoma-like cell line TCam-2 in comparison with human testicular biopsies

Fig. 5

Immunofluorescence labelling of selected junctional proteins in cultured FS1 and TCam-2 cells. (A–C) The immunosignal corresponding to N-cadherin is visible at the cell membranes (arrows) and cell extensions (dashed arrows) of FS1 cells and to a lesser extent in the cytoplasm (arrowheads). (D–F) A similar pattern occurs in TCam-2 cells with mainly the membranes (arrows) but also the cytoplasm (arrowheads) fluorescing. (G–I) A punctate fluorescent signal representing connexin 43 is localized at the cell membranes of FS1 cells (arrows), but areas of the cytoplasm are positive as well (arrowheads). (J–L) TCam-2 cells present only a weak reaction in the cytoplasm, especially perinuclear (arrowheads). (M–O) The fluorescent signal for connexin 45 is localized diffusely in the cytoplasm of FS1 cells, producing a dot-like pattern (arrowheads). (P–R) Similarly, in TCam-2 cells, the signal is distributed irregularly in the cytoplasm (arrowheads). The negative controls (inserts) do not show any specific labelling; only a weak fluorescence of the background can be observed. Scale bars = 20 μm

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BMC Cancer

ISSN: 1471-2407

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