Fig. 6

Combinated effect of raltitrexed and anlotinib on gene transcription in ESCC cells. mRNA levels of Bcl-2, MMP-9, Bax and Caspase-3 were measured by qPCR in either KYSE-30 (A-D) or TE-1 (E-H) cells after treated by 20 μM anlotinib, 2.5 μM raltitrexed, or both. Data indicate means + SD of three biological replicates. Student’s t test; ^*P < 0.05, ^**P < 0.01, ^***P < 0.01 (versus control); ^##P < 0.01, ^###P < 0.001 (versus 20 μM anlotinib or 2.5 μM raltitrexed)