Fig. 7

VDR directly activates LEF-1 expression in SW480 cells. a The diagram represents the LUC reporter construction containing three putative VDR binding sites (WRE-1, WRE-2 and WRE-3) in the LEF-1 promoter region (2000 bp upstream of the ATG) and four mutated LEF-1 promoters with only one WRE binding site or none. b The relative LUC activity of the constructs in A from the dual LUC reporter assay. c The percentage relative to input DNA for VDR ChIP was quantified. d The relative DNA levels of the three WRE binding sites from VDR ChIP were quantified and controlled by IgG. The data are represented as means ± SD, n ≥ 3, *P < 0.05, **P < 0.01