Fig. 6From: Vitamin D receptor prevents tumour development by regulating the Wnt/β-catenin signalling pathway in human colorectal cancerVDR interacts with β-catenin at the protein level, and VDR overexpression promotes the nuclear accumulation of β-catenin. a The Co-IP assay showed that the Myc antibody efficiently immunoprecipitated the β-catenin proteins controlled by the input. The initial biomasses were normalized to beta-Actin. b Western blotting analysis showed the accumulation of β-catenin in the cytoplasm and nucleus with VDR overexpression. GAPDH and CREB were used as protein controls. c The GST pull-down assay showed that the GST-β-catenin fusion proteins could pull down Myc-VDR efficiently. Beta-Actin was used as the protein controlBack to article page