Fig. 12

A The relative mRNA expression levels were measured using the fold-change in each protein relative to GAPDH. The data are expressed as the mean ± standard deviation. B Expression of ferroptosis-related protein was measured by western blot, The relative protein expression levels were quantified and measured using the fold-change in each protein relative to β-actin, *P < 0.05 and **P < 0.01 vs. hFOB1.19. C The knockdown efficiency of siRNA on five key genes was measured by westernblot, and wound healing assay results based on siRNA shows the migration ability differences. D Quantification of wound healing assay. E Cell viability assay by CCK-8. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. si-NC