Fig. 6

TAMs-derived HBEGF promotes proliferation and invasion of SW1990. a qRT-PCR evaluation of relative expressions of GRN, LGALS9, and HBEGF across three groups. b Line charts showing the levels of cell viability across the three groups (SW1990 alone, co-cultured SW1990, and co-cultured SW1990 with HBEGF blocking). The absorbance value at a wavelength of 450 nm was used as an indicator of cell viability. Data are presented as means ± SEM and analyzed with Student t-test (ns: non-significance; *P < 0.05; **P < 0.01). c Representative phase-contrast images of crystal violet stained SW1990 cells that invaded through the Matrigel matrix after 24 h of preprocessing (magnification 40×) across the three groups (SW1990 alone, co-cultured SW1990, and co-cultured SW1990 with HBEGF blocking). The quantitative analysis of invaded cells was performed by collecting five fields of view, and the data was presented as means ± SEM ( ***P < 0.001)