Fig. 1

Identification of macrophage heterogeneity. a t-SNE projection of 5408 myeloid cells from PDAC tumors and normal tissues, colored according to graph-based clusterings (left panel) or sample origin (right panel). b t-SNE plots showing normalized expression of S100A8, CD68, PRSS1, DERL3, CCR7, and CD1C, representative cell markers for specific cell types. c Dot plot depicting expression levels of representative marker genes across different myeloid subsets. d Hundred-percent bar chart illustrating the distributions of different macrophage subsets across PDAC samples. e Heatmap illustrating expressions of M1 and M2 markers across different macrophage subsets. pDC, plasmacytoid dendritic cell; cDC, conventional dendritic cell; t-SNE, t-distributed stochastic neighbor embedding