Fig. 2

Lipophilic statins potentiate pro-inflammatory responses in vitro. (a) Viability of HCT116-GFP cells in mono- and co-cultures 72 h after treatment with statins at a final concentration of 10 µM (n = 3). (b) Viability measured in HCT116-GFP cells in monoculture or co-cultures with PBMCs from three different donors, treated with DMSO vehicle (0.01%, 0.1%), dexamethasone (DEX) (10 µM), or one of the three statins mevastatin, simvastatin, and pitavastatin (1 µM, 10 µM) for 72 h. Data is shown as mean ± SEM (n = 3). Luminex MAGPIX system assessment of (c) IL-1β, (d) IL-10, (e) IFN-γ, and (f) TNF-α concentrations measured in co-culture supernatants collected after 24 h treatment. Data shown as mean ± SD from three independent experiments. One-way Anova with Dunnett’s multiple comparison test, compared to DMSO vehicle, *adjusted P-value < 0.05, **adjusted P-value < 0.01