Fig. 5

After the final tumor size measurement, splenocytes, collected from individual mice belonging to different experimental groups, were stimulated after 72 h of exposure to rCD44v (100 ng/µL), and the proliferative rates of mice lymphocyte cells were determined by MTT assay. Data represent the mean of stimulation index for 6 different mouse groups ± standard error. The difference in mean SI. Values between groups were assessed using ANOVA and comparisons were considered significant (Asterics show p < 0.05). Splenocytes were used without proximity to rCD44v (unstimulated cells) (a)

Increase of IFNγ, IL-17, and IL-4 secretion by splenocytes isolated from BALB/c mice immunized with vaccine. Splenocytes were cultured in vitro with rCD44v (100ng/µL). After being cultured for 3 days, the supernatants were harvested, and IFNγ, IL-17, and IL-4 releases were measured by ELISA assay. The rCD44v showed significantly high levels of IFNγ and IL-17-producing ability in comparison to the control groups (Asterias show P < 0.05) (b). The mean ± SD of triplicate determinations is shown