Fig. 2

(A) The spectrum of 9p24.1 alterations in CRC. Individual tumors (deletion, polysomy, or amplification) are depicted as columns on the x-axis and the percentage of tumor cells with 9p24.1 disomy (blue), deletion (green), polysomy (light pink), and/or amplification (red) is shown on the y-axis. (B) Percentage of tumor cells with residual 9p24.1 disomy in CRC is classified by 9p24.1 alterations, as represented as box-and-whisker plots. CRC with 9p24.1 deletion, polysomy, and amplification have significantly different percentages of residual tumor cells with normal (disomic) 9p24.1 copy numbers (amplification vs. deletion, p < 0.001; amplification vs. polysomy, p < 0.001, Kruskal-Wallis test). (C) Association of PD-L1 protein expression and 9p24.1 copy number alterations. Residual 9p24.1 disomy is depicted on the Y-axis; PD-L1 immunohistochemistry and H-score (in quartiles) is shown on the X-axis. Quartiles are indicated by dashed lines. A highly significant decrease in percentage of residual 9p24.1 disomic cells in CRC with a higher PD-L1 IHC H-score is shown. p = 0.034, Kruskal-Wallis test