Fig. 4

(A) Verification of transfection efficiency in the CAL27 cell line; (B) Detection of cell proliferation in the CAL27 cell line; (C) Verification of transfection efficiency in the SCC25 cell line; (D) Detection of cell proliferation in the SCC25 cell line; (E) Detection of cellular colony formation in the CAL27 cell line; (F) Detection of cellular colony formation in the SCC25 cell line; (G) Detection of cell cycle in the CAL27 cell line (n = 3); (H) Detection of cell cycle in the SCC25 cell line; (I) Detection of apoptosis in the CAL27 cell line (n = 3); (J) Detection of apoptosis in the SCC25 cell line (n = 3); (K) Detection of invasion in the CAL27 cell line (n = 3); (L) Detection of invasion in the SCC25 cell line (n = 3); (M) Detection of migration in the CAL27 cell line (n = 3); (N) Detection of migration in the SCC25 cell line (n = 3). C, blank control group; NC, mimics negative control group; M, mimics group; IN, inhibitor negative control group; I, inhibitor group. * P < 0.05 and ** P < 0.01 vs. Control group; # P < 0.05 and ## P < 0.01 vs. mimics / inhibitor negative control group