Fig. 2

Down regulation of IGF-1R expression and activation restored the inhibitory effect of sorafenib on the proliferation of HCC cells. (A, B) Western blot detected the expression level of IGF-1R and p-IGF1R in HCC cells. (C, D) HCC cells were exposed to each drug treatment and stained with EdU (×200). The cell proliferation rate was calculated as the percentage of EdU-positive nuclei in the total nuclei. (E, F) The cell clones in the six-well plate were stained with 0.1% crystal violet dye. The clones were counted, and representative photos of the clones were taken. (G) HCC cells were exposed to each drug treatment. The absorbance was measured, and the cell viability was calculated. Sorafenib (4 µM), IGF-1 (100 ng/mL), and PPP (100 nM). The statistics chart represents the mean ± SD determined in triplicate