Fig. 2

TGIF2 promotes EMT and metastasis of LUAD cells in vivo. (A) Bioluminescence imaging of pulmonary metastasis. Mice were intravenously injected with H1299-firefly luciferase stable cell strains and subjected to a bioluminescence test after 20 d. (B) Quantitation of bioluminescence signals in the indicated mice. Data are presented as the mean ± SEM (n = 6 mice per group). (C) H&E staining was used to analyze lung metastasis in the indicated mice. Scale bars, 250 μm. (D) Percentage of metastatic area in mouse lungs. (E) IHC analysis of E-cadherin and Vimentin in the indicated lung tumors (n = 4). Scale bars: 50 μm, upper panel. *, p < 0.05; **, p < 0.01; ***, p < 0.001. All data are representative of three repeated experiments