Fig. 5

Effects of silencing TAP1 expression on UVM cells In vitro. A PCR showed that siRNA successfully silenced the mRNA expression of TAP1; B CCK8 experiment showed that silencing TAP1 significantly inhibited the proliferation activity of C918; C WB experiment showed that siRNA successfully inhibited the protein expression of TAP1, and the protein expression of MMP9 and MMP2 also decreased; D Colony formation assay showed that silencing TAP1 significantly inhibited the growth activity of C918; E Apoptosis flow cytometry showed that the number of tumor cells apoptotic was significantly increased; F The cell cycle assay showed that tumor cell cycle was blocked in S phase; G-H Scratch and transwell experiments showed that the tumor cells in the siRNA group had significantly reduced ability to metastasize and migrate. The scale bar represents 50 μm in all the pictures