Fig. 8
EOAI can enhance the effect of cisplatin anti-tumor. A Plant the mice Lewis lung cell (LLC) into the side abdominal skin of the C57 mice. When the tumor reaches 100 mm3, start EOAI and CDDP treatment. There are 5 animals in each group. Measure the volume of tumors and mice weight every 3 days. After 21 days, the mouse was executed, and the growth curve was drawn. B The mice were sacrificed on the 21st day of tumor formation, and the tumor was exfoliated and photographed. C Evaluate the difference between the size of mice tumors in different therapeutic groups through the t-test with GraphPad Prism8 software. D Immunohistochemical staining (IHC) of tumor tissue in mice. IHC was performed on tumor tissues of mice and classified according to the degree of staining, and reprehensive pictures were captured. Light yellow indicates weak positivity ( +), brownish yellow indicates moderate positivity (+ +), and brownish-black indicates strong positivity (+ + +)