Fig. 6

Treatment with EOAI induced DNA damage in NSCLC cells. A The comet assay detected DNA damage. A549 and H460 cells were treated with DMSO or EOAI as indicated. 24 h later, cells were collected and detected by the comet assay as described in the Materials and methods section. Images were captured under fluorescence microscopy, represented pictures in the left panel, and the quantization of comet tails is shown in the right panel. B γ-H2AX foci were determined by immunofluorescence. A549 and H460 cells were treated with EOAI at indicated concentrations. γ-H2AX foci were determined by immunofluorescence. C The expression of γH2AX was determined by western blotting. A549 and H460 cells were treated with EOAI, and cell extracts were subjected to western blotting. GAPDH served as a loading control. D Relationship between DNA damage and cell cycle, autophagy, and apoptosis. A549 and H460 cells were treated with EOAI. Cells were collected at different time points and subjected to western blotting. The expression of γ-H2AX, LC3, total PARP and cleaved PARP, p21, p27, and in different time points was detected. GAPDH served as a loading control