Fig. 2

EOAI triggered G2/M cell cycle arrest in NSCLC cells. A549 and H460 cells were treated with EOAI as indicated for 24 h, followed by PI staining. Representative pictures were shown in panel (A). Distribution was analyzed and shown in panel (B). The t-test of GraphPad Prism8 software assessed the statistical significance of differences between groups in panel b. * The difference between the two groups was significant (P < 0.05). All data represent at least three independent experiments (n = 3; error bar, SD). C The expression of cell-cycle-related proteins was detected. A549 and H460 cells were subsequently treated with EOAI at indicated concentrations and subjected to western blotting. Use GAPDH as the loading control. All data were representative of at least three independent experiments