Fig. 5
From: Investigating the functional role of SETD6 in lung adenocarcinoma
The regulatory effect of SETD6 on NF-κB and Nrf2 signaling pathways. The protein levels of (A) intra or extranuclear P65, and (B) intra or extranuclear Nrf2, in control, SEDT6-3*Flag or SETD6-shRNA c transfected A549 cells were analyzed by Western blot. LaminB1 served as loading control for nuclear protein and GAPDH served as loading control for cytoplasm protein. C The protein levels of SETD6, Nrf2, Keap1, HO-1, SOD-1 were measured by Western blot. β-actin served as loading control. Protein bands were quantified by Quantity One 4.6.2 software analysis. *, P < 0.05 compared to control group; **, P < 0.01 compared to control group; #, P < 0.05 compared to SEDT6-3*Flag group; ##, P < 0.01 compared to SEDT6-3*Flag group