Fig. 2
From: Investigating the functional role of SETD6 in lung adenocarcinoma
The effect of SEDT6 overexpression with SEDT6-3*Flag transfection on A549 cell migration, colony formation and viability. A SETD6 protein levels were determined by western blot in control, plenti-CMV or SEDT6-3*Flag transfected A549 cells. β-actin served as loading control. Protein bands were quantified by Quantity One 4.6.2 software analysis. B Cell migration rate of control, plenti-CMV or SEDT6-3*Flag transfected A549 cells at indicated time points (0 h, 24 h, 48 h) were determined by wound healing assay. C Colony formation rate of control, plenti-CMV or SEDT6-3*Flag transfected A549 cells were determined by colony formation assay. D Cell viability of control, plenti-CMV or SEDT6-3*Flag transfected A549 cells were determined by MTT method. The cell viability was measured at different time (12, 24, 36, 48, 60, 72 h) and the percentage of survival of cells relative to controls was calculated. *, P < 0.05 compared to control group; **, P < 0.01 compared to control group; ##, P < 0.01 compared to plenti-CMV group