Fig. 4
DHODH inhibition reduced the level of O2.−, ATP, and basal OCR in SOD2 knockdown cells. Effect of DHODH knockdown on OCR in SOD2 depleted and control cells (A) without radiation and (B) with radiation. Fluorescence microscopy images and bar plots showing the effect of (C) DHODH knockdown and (D) 5 µM BQ on O2.− fluorescence intensity in control and SOD2 knockdown cells. E Effect of DHODH knockdown on ATP level in SOD2 depleted and control 5-8F and CNE 2 cells. F Effect of 5 µM BQ on ATP level in SOD2 knockdown and control 5-8F and CNE 2 cells. Radiation dosage: 4 Gy. n ≥ 3 independent repeats. *P < 0.05, **P < 0.01, ***P < 0.001