Fig. 5

The effect of MICAL1 on Rac1 activation and renal clear cell carcinoma cell migration. a Caki-1 and 786-O cells were transfected with control siRNA or siRNA specifically targeting MICAL1 (siMICAL1) for 48 h, then total protein extracts from those cells were analyzed for MICAL1 expression. Western blot bands corresponding to MICAL1 were quantified and normalized against GAPDH. The statistical graph from Caki-1 cells was shown in Fig. 1A panel on the right. b After knockdown of MICAL1 for 48 h, the proliferation rates of Caki-1 and 786-O cells were assessed by CCK8 assays. (C&D) After knockdown of MICAL1 for 36 h, the migratory rates of these cells were assessed by Transwell c and wound healing assays d. e Levels of GTP-Rac1 in MICAL1-knockdown Caki-1 cells were assessed. *p < 0.05, **p < 0.01