Fig. 2

Cytotoxic activity of GemHCl and MFU on MiaPaCa-2 and Panc-1 cells after 48 h incubation and stability studies of MFU. (a) and (b) 2-D cultured cells showing GemHCl and MFU-treated MiaPaCa-2 and Panc-1 cells, respectively; (c) and (d) 3-D cultured cells) showing GemHCl and MFU-treated MiaPaCa-2 and Panc-1 cells, respectively. Fluorescent images, (e) and (f) showing 3-D organoids of MiaPaca-2 and Panc-1 cells treated with GemHCl and (g) and (h) showing 3-D organoids of MiaPaca-2 and Panc-1 cells treated with MFU. (i) Shows percent of MFU remaining after treatment of MiaPaca-2 and Panc-1 cells over 24 h; (j) showing percent of intact MFU remaining after exposure to human liver microsomes over 2 h