Fig. 8

Cytosolic calcium participated in conditioned medium-mediated NF-κB signal and LPS-mediated canonical NF-κB signal. BMDMs treated by LL-2-CM and CMT-64-CM with or without BAPTA-AM for 2 h were collected for western blot. Canonical NF-κB (p-p65 and p65), non-canonical NF-κB (p-RelB, RelB, and p100/p52), and overall NF-κB (p-IKK, IKK \(\mathrm{\alpha }\), and IKK \(\upbeta\)) were shown and calculated A-I. LPS elicited canonical NF-κB in BMDMs, which was suppressed by BAPTA-AM J-L. Full-length blots are presented in Supplementary Fig. 5 and Supplementary Fig. 6. P values were calculated by one-way ANOVA and Tukey test