Fig. 8

Higher RAB1A expression depletes miR-19a-3p-mediated suppression of HCC cells. A qRT-PCR was performed to analyze the relative levels of RAB1A in the obtained stage I-II and III-IV HCC tissue specimens. B Kaplan-Meier survival analysis was performed for HCC patients according to the levels of RAB1A. C Relative intracellular RAB1A mRNA levels in Huh7 and HA22T cells after treatment with RAB1A overexpression vector (RAB1A). D CCK-8 assays were operated to determine the changes of HCC cell viability after transfection with mimic nc + vector, mimic + vector, and mimic + RAB1A. E, F Apoptosis rates of Huh7 and HA22T cells were detected by flow cytometry. Data obtained by flow cytometry (left, E) and data analysis images (right, F) are shown. G, H Transwell assay was manipulated to assess cell migration and invasion abilities. The number of migrating and invasive cells (right) was calculated and the landmark images obtained by transwell (left) are shown. Scale bar, 50 μm. I Images represented TUNEL staining of HCC cells after transfection with mimic nc + vector, mimic + vector, and mimic + RAB1A. TUNEL positive cell ratio was also demonstrated (right panel). Scale bar, 50 μm. *** P < 0.001, ^## P < 0.01, ^### P < 0.001. *** P < 0.001 indicates compared to vector group and mimic nc + vector; ^## P < 0.01 and ^### P < 0.001 indicate compared to mimic + vector group