Fig. 2

Hepatocellular carcinoma-derived exosomes induce tumor cell growth, migration and invasion in vitro. A After 24 h of treatment with or without HCC cell-derived exosomes, Huh7 and HA22T cell activities were examined separately by CCK-8 assay to characterize the altered growth activity. B Annexin V/PE and 7-AAD staining and flow cytometry were performed on Huh7 and HA22T cells in the presence or absence of exosomes. C, D Transwell analysis was performed on Huh7 and HA22T cells after co-incubation with or without HCC cell-derived exosomes for 24 h to assess their migratory (C) and invasive (D) abilities. Scale bar, 50 μm. E TUNEL analysis illustrated the effect of exosome treatment on HCC cell apoptosis. Scale bar, 50 μm. *** P < 0.001. *** P < 0.001 indicates compared to control group