Fig. 5

MYBL2 regulates IMPDH1 expression at transcriptional level. A ChIP-seq tracks showing MYBL2 binding to the promoter region of IMPDH1 in HepG2 cells. Data was from ENCODE Project. B mRNA abundance of IMPDH1 in HepG2 cells expressing lentiCRISPRv2 empty vector (EV) or gRNA targeting MYBL2 (MYBL2 KO) and MYC (MYC KO). ** P < 0.01. C, D. Protein abundance of MYC, MYBL2, and IMPDH1 in HepG2 cells in B. *** P < 0.001. E. qPCR for the IMPDH1 promoter after ChIP with anti-MYC, anti-MYBL2 antibodies or IgG control in HepG2 cells