Fig. 4

MYBL2 reprograms purine metabolism and is required for HCC tumor growth. A Xenograft models were established in nude mice with HepG2 cells expressing lentiCRISPRv2 empty vector (EV) or gRNA targeting MYBL2 (MYBL2 KO). Tumor sizes were decreased in tumors post MYBL2 knockout. B Protein abundance of MYBL2 in HepG2 cells expressing lentiCRISPRv2 empty vector (EV) or gRNA targeting MYBL2 (MYBL2 KO). C Metabolic profiles difference between HepG2 cells and MYBL2 knockout HepG2 cells. Variable importance in the projection (VIP) score (VIP > 1.0, metabolites with VIP > 1.2 are shown) was shown. The bar indicated relative metabolite abundance, with red representing metabolite accumulation. D Principal component analysis for the extracts of 3 HepG2 cells with empty vector and 3 HepG2 cells with MYBL2 knockout. No overlap was found. E Relative abundance of GMP, IMP and AMP in HepG2 cells expressing empty vector or CRISPR/Cas9-mediated knockout of MYBL2. *** P < 0.001, * P < 0.05