Fig. 3
From: Oncogenic KRAS alters splicing factor phosphorylation and alternative splicing in lung cancer
A large-scale transcriptome screen as a platform for splicing discovery. A Experimental workflow for RNA-seq analysis of lung adenocarcinoma genes and variants. B Transcriptome profiles of cells expressing different gene constructs. Dimensionality reduction performed using t-distributed stochastic neighbor embedding (t-SNE) [67]. Each point represents an experimental replicate. Genes and alleles of particular interest are colored and labeled. Controls are circles colored in black. Circle = wild-type allele. Diamond = variant allele. C Gene set analysis with GOseq of up- or down-regulated transcripts in MYC, KRAS, FBXW7, and negative control cells. Hallmark gene sets from mSigDB