Fig. 2

Oncogenic KRAS regulates alternative splicing in lung epithelial cell. A Schematic of the inclusion or skipping events of a cassette exon. B Differentially spliced exons in KRAS^G12V and KRAS^Q61H cells compared to KRAS^WT cells. PSI = Percent Spliced In. Red = exons promoted at least 10% more in KRAS^WT than KRAS^mut. Blue = exons promoted at least 10% more in KRAS^mut than KRAS^WT. FDR < 0.05. C Overlap of differentially spliced exons in KRAS^G12V vs KRAS^WT and KRAS^Q61H vs KRAS^WT. P-value calculated by modeling skipped exon events as a hypergeometric distribution. D Read coverage plots of skipped exon events in MOK and NT5C2, normalized by total RNA-seq library size and RNA composition. Left axis = percent coverage relative to region. Right axis = absolute read coverage